MABSol® Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (TNA-H8211) is expressed from human HEK293 cells. It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). It is the biotinylated form of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (TNA-H4211).
Predicted N-terminus: Val 77
The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
The biotin to protein ratio is 1-3 as determined by the HABA assay.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by reduced SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) was more than 95% in HP-SEC, and around 48-65 kDa verified by SEC-MALS.
Immobilized Humira at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (QC tested).
Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (active trimer) (MALS verified) (Cat. No. TNA-H8211) with a linear range of 0.4-6 ng/mL (Routinely tested).
Authors: D Lebert, et al
Journal: US20190011455A1 43475
Application: Affinity chromatography
Authors: Lebert Dorothée, et al
Journal: US20180321253A1 43412
Application: Mass Spectrometry ImmunoAffinity
Authors: Yu Bo, et al.
Journal: US20180313834A1 43405
Application: Flow Cytometry
Quantification of active infliximab in human serum with liquid chromatography-tandem mass spectrometry using a tumor necrosis factor alpha -based pre-analytical sample purification and a stable isotopic labeled infliximab bio-similar as internal standard: A target-based, sensitive and cost-effective method
Authors: El Amrani M, et al.
Journal: J Chromatogr A 2016
Authors: P Sabhachandani, et al.
Journal: Microchim Acta 2015
Application: Microsphere-based assay
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.