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Human TRAIL R1 / DR4 / TNFRSF10A Protein, His Tag

分子别名(Synonym)

TNFRSF10A,TRAIL-R1,CD261,APO2,DR4

表达区间及表达系统(Source)

Human TRAIL R1, His Tag (TR1-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 239 (Accession # NP_003835).

Predicted N-terminus: Ala 24

蛋白结构(Molecular Characterization)

Online(Ala 24 - Asn 239) NP_003835

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.7 kDa. The protein migrates as 19-23 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TRAIL R1, His Tag (Cat. No. TR1-H5228) SDS-PAGE gel

Human TRAIL R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TRAIL R1, His Tag (Cat. No. TR1-H5228) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 2-20 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human TRAIL R1, His Tag (Cat. No. TR1-H5228) on HIS1K Biosensor, can bind Human TRAIL with an affinity constant of 0.583 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TRAILR1 / TNFRSF10A contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAILR-1 / DR4 / CD261 promotes the activation of NF-kappa-B.

 

前沿进展

 
货号/价格

TR1-H5228-100ug / ¥2600

TR1-H5228-1mg (500ug X 2) / ¥16000

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