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Human TNFR1 / CD120a / TNFRSF1A Protein, Fc Tag

分子别名(Synonym)

TNFR1,CD120a,TNFRSF1A,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR55,TNFR60,p55,p60

表达区间及表达系统(Source)

Human TNFR1, Fc Tag (TN1-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Thr 211 (Accession # NP_001056).

Predicted N-terminus: Ile 22

蛋白结构(Molecular Characterization)

Online(Ile 22 - Thr 211) NP_001056

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 47.9 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TNFR1, Fc Tag (Cat. No. TN1-H5251) SDS-PAGE gel

Human TNFR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TNF-alpha, Tag Free, low endotoxin (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

背景(Background)

Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).

 

前沿进展

 
货号/价格

TN1-H5251-100ug / ¥2500

TN1-H5251-1mg (250ug X 4) / ¥16000

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