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Human TIGIT Protein, His Tag (MALS verified)

分子别名(Synonym)

TIGIT,VSIG9,VSTM3

表达区间及表达系统(Source)

Human TIGIT, His Tag (MALS verified) (TIT-H52H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

Predicted N-terminus: Met 22

蛋白结构(Molecular Characterization)

Online(Met 22 - Pro 141) Q495A1-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TIGIT, His Tag (MALS verified) (Cat. No. TIT-H52H3) SDS-PAGE gel

Human TIGIT, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human TIGIT, His Tag (MALS verified) (Cat. No. TIT-H52H3) MALS images

The purity of Human TIGIT, His Tag (MALS verified) (Cat. No. TIT-H52H3) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H3) at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 8-128 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human TIGIT, His Tag (Cat. No. TIT-H52H3) at 1 μg/mL (100 μL/well) can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.2-2.5 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, His Tag (Cat. No. TIT-H52H3): Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Anti-Human TIGIT MAb (Mouse IgG1) on AMC Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.72 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 0.732 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS assay shows that Human TIGIT, His Tag (Cat. No. TIT-H52H3) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.3 μg/mL (Routinely tested).

Protocol

 

文献引用(Citations)

背景(Background)

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

 

前沿进展

 
货号/价格

TIT-H52H3-100ug / ¥3500

TIT-H52H3-1mg / ¥23100

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TIGIT靶点信息
英文全称:T cell immunoglobulin and ITIM domains
中文全称:T细胞免疫球蛋白ITIM结构域
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:8详情
最高研发阶段:临床三期
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