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Human TGF-beta RII / TGFBR2 Protein, Fc Tag

分子别名(Synonym)

TGFBR2,TGFR2,TbetaR-II,TGFβR2

表达区间及表达系统(Source)

Human TGFBR2, Fc Tag (TG2-H5252) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asp 159 (Accession # NP_003233).

Predicted N-terminus: Thr 23

蛋白结构(Molecular Characterization)

Online(Thr 23 - Asp 159) NP_003233

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 42.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) SDS-PAGE gel

Human TGFBR2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TGFB1, His Tag (Cat. No. TG1-H524x) at 1 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) with a linear range of 0.3-2 ng/mL (QC tested).

Protocol

文献引用(Citations)

背景(Background)

TGF-beta receptor type-2 (TGFBR2 or TGFR-2) is also known as TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TbetaR-II, TGFβR2, which is a homodimer or heterohexamer, belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. TGFR2 / TGFBR2 binds TGF-β1 / TGFB1 and TGF-β3 / TGFB3 with high affinity and TGF-β2 / TGFB2 with a much lower affinity. This type I I receptor forms a heterodimeric complex with type I receptor and is essential for signal transduction. Upon ligand binding, the TGFR2 autophosphorylates its cytoplasmic domain and subsequently phosphorylates the downstream molecules which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.

 

前沿进展

 
货号/价格

TG2-H5252-100ug / ¥3150

TG2-H5252-1mg (500ug X 2) / ¥19800

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