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Human TFPI Protein, His Tag (active enzyme)

分子别名(Synonym)

TFPI,LACI,TFPI1,EPI,TFI

表达区间及表达系统(Source)

Human TFPI, His Tag (TFI-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).

Predicted N-terminus: Asp 29

蛋白结构(Molecular Characterization)

Online(Asp 29 - Lys 282) NP_006278.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human TFPI, His Tag (Cat. No. TFI-H5226) SDS-PAGE gel

Human TFPI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate. The IC50 value is <0.50 nM, as measured under the described conditions (QC tested).

Protocol

 

背景(Background)

Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

 

前沿进展

 
货号/价格

TFI-H5226-50ug / ¥3100

TFI-H5226-1mg / ¥22000

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TFPI靶点信息
英文全称:Tissue factor pathway inhibitor
中文全称:组织因子通道抑制剂
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床三期
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