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Biotinylated Human SOST / Sclerostin Protein, His Tag, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

SOST,VBCH

表达区间及表达系统(Source)

MABSol® Biotinylated Human SOST, His Tag, primary amine labeling (SOT-H8245) is expressed from human HEK293 cells. It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1). It is the biotinylated form of Human SOST, His Tag (HST-H5245).

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

Online(Gln 24 - Tyr 213) AAI01087.1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 4-6 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human SOST, His Tag, primary amine labeling (Cat. No. SOT-H8245) SDS-PAGE gel

Biotinylated Human SOST, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

背景(Background)

Sclerostin (SOST) also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.

 

前沿进展

 
货号/价格

SOT-H8245-25ug / ¥2900

SOT-H8245-200ug / ¥12500

开发建议
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SOST靶点信息
英文全称:Sclerostin
中文全称:硬化蛋白
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:4详情
最高研发阶段:批准上市
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