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Human SIRP gamma / CD172g Protein, Fc Tag

分子别名(Synonym)

SIRPG,CD172g,SIRPB2,SIRP-gamma,SIRP-b2,SIRP-beta-2,Cripto-1

表达区间及表达系统(Source)

Human SIRP gamma, Fc Tag (SIG-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532).

Predicted N-terminus: Glu 29

蛋白结构(Molecular Characterization)

Online(Glu 29 - Ser 364) AAH64532

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 63.7 kDa. The protein migrates as 68-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human SIRP gamma, Fc Tag (Cat. No. SIG-H5253) SDS-PAGE gel

Human SIRP gamma, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP gamma, Fc Tag (Cat. No. SIG-H5253) with a linear range of 0.01-0.156 μg/mL (QC tested).

点击申请Protocol

背景(Background)

Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.

 

前沿进展

 
货号/价格

SIG-H5253-100ug / ¥2500

SIG-H5253-1mg (500ug X 2) / ¥14400

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