分子别名（Synonym）
PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
表达区间及表达系统（Source）
Biotinylated Human PD-L1, Avitag™,His Tag (PD1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Predicted N-terminus: Phe 19
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
应用说明（Application）
PD1-H82E5 works best for experiments that test the binding between PD-L1 and candidate antibodies, such as biopanning and other relevant assays.
This product is NOT suitable for testing PD1-PDL1 binding. For this type of application, we strongly recommend you to choose PD1-H82F3 as an alternative.
标记（Biotinylation）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Biotin:Protein Ratio)
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human PD-L1, Avitag™,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human PD-L1, Avitag™,His Tag (Cat. No. PD1-H82E5) was more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) (Cat. No. PD1-H5255) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well)on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-Human PD-L1 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) (Cat. No. PD1-H82E5) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
文献引用（Citations）
-
Authors: Son S, et al
Journal: J Drug Target 2019
Application: ELISA
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Comprehensive in vitro characterization of PD-L1 small molecule inhibitors
Authors: Ganesan A, et al
Journal: Sci Rep 2019
Application: ELISA
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Anti-pd-1 antibodies and methods of use thereof
Authors: Yasmina Noubia Abdiche, et al.
Journal: US20160159905A1 2016
Application: Flow Cytometry
背景（Background）
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
