Human PTH1R, His Tag (PTR-H5222) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Gly 188 (Accession # Q03431-1).
Predicted N-terminus: Asp 27
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.6 kDa. The protein migrates as 27-43 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Less than 1.0 EU per μg by the LAL method.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PTH1R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Parathyroid hormone/parathyroid hormone-related peptide receptor is also known as Parathyroid hormone 1 receptor (PTH1R), PTHR1, PTH/PTHrP type I receptor (PTH/PTHr receptor), which belongs to the G-protein coupled receptor 2 family. PTH1R / PTHR1 is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.