PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
Human PD-L2, Fc Tag (HPLC verified) (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
Predicted N-terminus: Leu 20
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PD-L2, Fc Tag (HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) was greater than 90% as determined by SEC-HPLC.
Loaded Human PD-1, His Tag (HPLC verified) (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
活性（Bioactivity）-Cell based assay
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).
Authors: Wang Aijun, et al.
Journal: US20170355770A1 2017
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.