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Human PD-L1 / B7-H1 Protein, Fc Tag, low endotoxin

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

Human PD-L1, Fc Tag (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

PD-L1(Phe 19 - Arg 238) NP_054862.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) SDS-PAGE gel

Human PD-L1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) HPLC images

The purity of Human PD-L1, Fc Tag (HPLC-verified)(Cat.# PD1-H5258) was greater than 95% as determined by SEC-HPLC.

 

活性(Bioactivity)

Human PD-L1, Fc Tag (Catalog # PD1-H5258) ELISA bioactivity

Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 5 μg/mL ( 100 μl/well ) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 1-50 ng/mL (Routinely tested).

点击申请Protocol

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.31-1.25 μg/mL (QC tested).

点击申请Protocol

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

点击申请Protocol

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

FACS analysis shows that the binding of PD-L1 to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-hPD-L1 antibody. The concentration of PD-L1 used is 10 μg/mL. The IC50 is 12.92 μg/mL (Routinely tested).

点击申请Protocol

Human PD-L1, Fc Tag (Cat. No. PD1-H5258) ELISA bioactivity

Flow Cytometry assay shows that recombinant human PD-L1 (Cat. No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 μg/mL (Routinely tested).

点击申请Protocol

 

背景(Background)

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

参考文献(References)

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


货号/价格

PD1-H5258-100ug / ¥2900

PD1-H5258-1mg (500ug x 2) / ¥21000

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