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Human PD-L1 / B7-H1 Protein, His Tag (HPLC-verified)

分子别名(Synonym)

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统(Source)

Human PD-L1, His Tag (HPLC-verified) (PD1-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ).

Predicted N-terminus: Phe 19

蛋白结构(Molecular Characterization)

Online(Phe 19 - Arg 238) NP_054862.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.0 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) SDS-PAGE gel

Human PD-L1, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-HPLC

Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) HPLC images

The purity of Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb with a linear range of 0.1-3 ng/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-AlphaLISA

Biotinylated Human AlphaLISA

Biotinylated Human PD-1, Fc Tag (Cat. No. PD1-H82F1) at 1 μg/mL (5 μL/well) can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

点击申请Protocol

 

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (QC tested).

点击申请Protocol

 
 

文献引用(Citations)

背景(Background)

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

 

前沿进展

货号/价格

PD1-H5229-100ug / ¥3000

PD1-H5229-1mg / ¥20000

文档
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PD-L1靶点信息
英文全称:Programmed death-ligand 1
中文全称:程序性死亡配体1
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:24详情
最高研发阶段:批准上市
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