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Human PD-1 / PDCD1 Protein, Fc Tag, low endotoxin (HPLC-verified)

分子别名(Synonym)

PDCD1,PD1,CD279,SLEB2

表达区间及表达系统(Source)

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (PD1-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).

N-terminal Sequence Analysis: Leu 25

蛋白结构(Molecular Characterization)

Online(Leu 25 - Gln 167) NP_005009.2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 42.6 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) SDS-PAGE gel

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) HPLC images

The purity of Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human PD-L1, His Tag, Avi Tag (recommended for biopanning) (Cat. No. PD1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2μg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) with a linear range of 0.1-3 ng/mL (QC tested).

点击申请Protocol

Biotinylated Human SPR

Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc Tag, Avi Tag (Cat. No. PD2-H82F6) with a linear range of 0.5-16 ng/mL. (Routinely tested).

点击申请Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human PD-L2, His Tag (Cat. No. PD2-H82E8) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2μg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).

点击申请Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

点击申请Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) on ProteinA Biosensor, can bind Human PD-L1, His Tag (HPLC & BLI verified) (Cat. No. PD1-H5229) with an affinity constant of 5.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

点击申请Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/mL (Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

 

前沿进展

货号/价格

PD1-H5257-100ug / ¥3000

PD1-H5257-1mg / ¥20000

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PD-1靶点信息
英文全称:Programmed cell death protein 1
中文全称:细胞程序性死亡-1
种类:Homo sapiens
上市药物数量:5详情
临床药物数量:58详情
最高研发阶段:批准上市
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