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Human PCSK9 Protein, His Tag

分子别名(Synonym)

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表达区间及表达系统(Source)

Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).

Predicted N-terminus: Gln 31

蛋白结构(Molecular Characterization)

Online(Gln 31 - Gln 692) Q8NBP7-1

This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.1 kDa respectively. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>97% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 24 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human PCSK9, His Tag (Cat. No. PC9-H5223) SDS-PAGE gel

Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5254) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 20-313 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 4.39 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性(Bioactivity)-FACS

Biotinylated Human FACS

FACS analysis shows that Human PCSK9, His Tag (Cat. No. PC9-H5223) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.2857-0.4668 μg/mL.

Protocol

 

文献引用(Citations)

背景(Background)

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

 

前沿进展

 
货号/价格

PC9-H5223-50ug / ¥3500

PC9-H5223-1mg (500ug X 2) / ¥23000

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PCSK9靶点信息
英文全称:Proprotein convertase subtilisin kexin type 9
中文全称:前蛋白转化酶枯草杆菌蛋白酶Kexin-9
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:28详情
最高研发阶段:批准上市
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