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 >  Protein>OX40 >OX0-H5224

Human OX40 / TNFRSF4 / CD134 Protein, His Tag (MALS verified)

分子别名(Synonym)

TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L

表达区间及表达系统(Source)

Human OX40 Protein, His Tag (OX0-H5224) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # P43489-1 ).

Predicted N-terminus: Leu 29

Request for sequence

蛋白结构(Molecular Characterization)

OX40 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 22.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

OX40 SDS-PAGE

Human OX40 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

OX40 SEC-MALS

The purity of Human OX40 Protein, His Tag (Cat. No. OX0-H5224) is more than 85% and the molecular weight of this protein is around 32-44 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human OX40 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

OX40 ELISA

Immobilized Human OX40 Protein, His Tag (Cat. No. OX0-H5224) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, Fc,Avitag (Cat. No. CT4-H82F3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

OX40 BLI

Loaded Human OX40 Ligand, His Tag (Cat. No. OXL-H52Q8) on AR2G Biosensor, can bind Human OX40 Protein, His Tag (Cat. No. OX0-H5224) with an affinity constant of 0.15 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

OX40 BLI

Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind Human OX40 Protein, His Tag (Cat. No. OX0-H5224) with an affinity constant of 6.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.

文献引用(Citations)

 

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OX40靶点信息
英文全称:Tumor necrosis factor receptor superfamily member 4
中文全称:肿瘤坏死因子受体超家族成员4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:27详情
最高研发阶段:临床三期
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