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Human Osteoprotegerin / TNFRSF11B Protein, Fc Tag

分子别名(Synonym)

TNFRSF11B,OCIF,OPG,Osteoprotegerin

表达区间及表达系统(Source)

Human Osteoprotegerin, Fc Tag (TNB-H5259) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537).

Predicted N-terminus: Glu 22

蛋白结构(Molecular Characterization)

Online(Glu 22 -  Leu 401) NP_002537

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 70.2 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) SDS-PAGE gel

Human Osteoprotegerin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 4-16 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human TRAIL, His Tag on HIS1K Biosensor, can bind Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) with an affinity constant of 1.65 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 2.32 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

 

前沿进展

 
货号/价格

TNB-H5259-100ug / ¥1800

TNB-H5259-1mg / ¥12000

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