登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>NME1 >NM1-H5147

Human NME1 / NDKA / NM23-H1 Protein, His Tag

分子别名(Synonym)

NME1,NDP kinase A,NDPA,GAAD,NM23-H1

表达区间及表达系统(Source)

Human NME1, His Tag (NM1-H5147) is expressed from E. coli cells. It contains AA Ala 2 - Glu 152 (Accession # AAH00293 ).

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

NME1 Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 18.0 kDa. The protein migrates as 17-18 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NME1 SDS-PAGE

Human NME1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%.

 

背景(Background)

Nucleoside diphosphate kinase A, a member of the NDK family, is also known as NME1, NDP kinase A (NDPA), granzyme A-activated DNase (GAAD), metastasis inhibition factor nm23 (NM23-H1) and tumor metastatic process-associated protein. NME1 plays a major role in the synthesis of nucleoside triphosphates other than ATP. NME1 is also involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Furthermore, NME1 is required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, NME1 works in concert with TREX1. NME1 nicks one strand of D and TREX1 removes bases from the free 3 end to enhance D damage and prevent D end reannealing and rapid repair.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定