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Human NKp30 / NCR3 / CD337 Protein, Fc Tag

分子别名(Synonym)

NCR3,CD337,NKp30,1C7,LY117,MALS

表达区间及表达系统(Source)

Human NKp30, Fc Tag (NC3-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Gly 135 (Accession # AAH52582).

Predicted N-terminus: Leu 19

蛋白结构(Molecular Characterization)

Online(Leu 19 - Gly 135) AAH52582

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 39.5 kDa. The protein migrates as 50-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human NKp30, Fc Tag (Cat. No. NC3-H5259) SDS-PAGE gel

Human NKp30, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human B7-H6, His Tag (Cat. No. B76-H52H8) at 10 μg/mL (100 μL/well) can bind Human NKp30, Fc Tag (Cat. No. NC3-H5259) with a linear range of 2-78 ng/mL (QC tested).

Protocol

背景(Background)

Natural cytotoxicity triggering receptor 3 (NCR3) is also known as Activating natural killer receptor p30, Natural killer cell p30-related protein (NK-p30), CD antigen CD337, 1C7, LY117. NCR3 /CD337 /NKp30 belongs to the natural cytotoxicity receptor (NCR) family. NCR3 /NKp30 contains one Ig-like (immunoglobulin-like) domain. NCR3 /NKp30 is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. NCR3 is homodimer in the unliganted form. NCR3 interacts with CD3Z. NCR3 interacts with and is activated by binding to NCR3LG1 or BAG6. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.

 

前沿进展

 
货号/价格

NC3-H5259-100ug / ¥2800

NC3-H5259-1mg / ¥17000

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