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Biotinylated Human Mesothelin / MSLN (296-580), Fc Tag, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

MABSol® Biotinylated Human Mesothelin (296-580), Fc Tag (MSN-H826x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272). It is the biotinylated form of Human Mesothelin (296-580), Fc Tag (MSN-H526x).

蛋白结构(Molecular Characterization)

Online(Glu 296 - Gly 580) AAH09272

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 3-5 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human Mesothelin, Fc Tag (Cat. No. MSN-H826x) SDS-PAGE gel

Biotinylated Human Mesothelin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Anti-Human MSLN Mab at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin, Fc Tag (Cat. No. MSN-H826x) with a linear range of 0.5-8 ng/mL (QC tested).

Protocol

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-MSLN CAR Expression

CAR

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human MSLN (296-580), Fc Tag (Cat. No. MSN-H826x, 3 µg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human MSLN (296-580), Fc Tag (Cat. No. MSN-H826x).

Protocol

 

文献引用(Citations)

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
货号/价格

MSN-H826x-25ug / ¥2900

MSN-H826x-200ug / ¥12500

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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:15详情
最高研发阶段:临床二期
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