膜杰作
Star Staining
分子别名（Synonym）
PVRL1,Nectin-1,CD111,CLPED1,ED4,HIgR,HVEC,OFC7,PRR,PRR1,PVRR,PVRR1,SK-12
表达区间及表达系统（Source）
Human Nectin-1, His Tag (PV1-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 334 (Accession # NP_002846).
Predicted N-terminus: Gln 31
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.0 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Nectin-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with a linear range of 0.078-1.25 μg/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.263 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Poliovirus receptor-related protein 1 (PVRL1) is also known as Herpes virus entry mediator C (HveC), Herpesvirus Ig-like receptor (HIgR), Nectin-1, CD antigen CD111, PVRL1 / Nectin-1 / CD111 belongs to the nectin family. PVRL1 / Nectin-1 is a membrane protein with three extracellular immunoglobulin domains, a single transmembrane helix and a cytoplasmic tail. The protein can mediate Ca2+-independent cellular adhesion further characterizing it as IgSF cell adhesion molecule (IgSF CAM). PVRL1 is adhesion molecule found in a wide range of tissues. PVRL1 / Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
