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Human M-CSF R / CSF1R / CD115 Protein, His Tag (HPLC verified)

分子别名(Synonym)

CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR

表达区间及表达系统(Source)

Human M-CSF R, His Tag (CSR-H5228) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2).

Predicted N-terminus: Ile 20

蛋白结构(Molecular Characterization)

Online(Ile 20 - Glu 512) NP_005202.2

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>96% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human M-CSF R, His Tag (Cat. No. CSR-H5228) SDS-PAGE gel

Human M-CSF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 96%.

SEC-HPLC

Human M-CSF R, His Tag (Cat. No. CSR-H5228) HPLC images

The purity of Human M-CSF R, His Tag (Cat. No. CSR-H5228) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human M-CSF R, His Tag (Cat. No. CSR-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 2-31 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 378 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

 

前沿进展

 
货号/价格

CSR-H5228-200ug / ¥2300

CSR-H5228-1mg (250ug X 4) / ¥9800

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