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Human LILRB4 / CD85k / ILT3 Protein, Fc Tag (SPR verified)

分子别名(Synonym)

LILRB4,ILT3,LIR5,CD85K,HM18

表达区间及表达系统(Source)

Human LILRB4, Fc Tag (SPR verified) (LI4-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309).

Predicted N-terminus: Gln 22

蛋白结构(Molecular Characterization)

Online(Gln 22 - Glu 259) AAH26309

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 52.9 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.0. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human LILRB4, Fc Tag (SPR verified) (Cat. No. LI4-H5259) SDS-PAGE gel

Human LILRB4, Fc Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human LILRB4, Fc Tag (SPR verified) (Cat. No. LI4-H5259) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human ANGPTL7, His tag (Cat. No. AN7-H52H5) with an affinity constant of 71.6 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

 

前沿进展

 
货号/价格

LI4-H5259-50ug / ¥3500

LI4-H5259-1mg / ¥22000

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