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Human Jagged 1 / JAG1 Protein, His Tag

分子别名(Synonym)

CD339,JAG1,Jagged1,hJ1,JAGL1

表达区间及表达系统(Source)

Human Jagged 1, His Tag (JA1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 34 - Ser 1046 (Accession # P78504-1).

Predicted N-terminus: Gln 34

蛋白结构(Molecular Characterization)

Online(Gln 34 - Ser 1046) P78504-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 112.3 kDa. The protein migrates as 130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Jagged 1, His Tag (Cat. No. JA1-H52H9) SDS-PAGE gel

Human Jagged 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human Jagged 1, His Tag (Cat. No. JA1-H52H9) immobilized on CM5 Chip can bind Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) with an affinity constant of 5.13 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Protein jagged-1 (JAG1) is also known as Jagged1, hJ1, JAGL1 and CD339, which is a single-pass type I membrane protein containing one DSL domain and 15 EGF-like domains. JAG1 is widely expressed in adult and fetal tissues,the expression of JAG1 is up-regulated in cervical squamous cell carcinoma and also expressed in bone marrow cell line HS-27a.JAG1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. JAG1 is involved in early and late stages of mammalian cardiovascular development. JAG1 inhibits myoblast differentiation by similarity and enhances fibroblast growth factor-induced angiogenesis (in vitro).

 

前沿进展

 
货号/价格

JA1-H52H9-50ug / ¥2900

JA1-H52H9-1mg (500ug X 2) / ¥22000

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Jagged 1靶点信息
英文全称:Protein jagged-1
中文全称:Jagged-1蛋白
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
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