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Human IL-7 R alpha / CD127 Protein, Fc Tag

分子别名(Synonym)

IL7Ra,CD127

表达区间及表达系统(Source)

Human IL-7 R alpha, Fc Tag (ILA-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1).

Predicted N-terminus: Glu 21

蛋白结构(Molecular Characterization)

Online(Glu 21 - Gly 236) P16871-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.0 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-7 R alpha, Fc Tag (Cat. No. ILA-H5258) SDS-PAGE gel

Human IL-7 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Fc Tag (Cat. No. ILA-H5258) with a linear range of 1-16 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Captured Human IL-7 R alpha, Fc Tag (Cat. No. ILA-H5258) on CM5 chip via anti-human IgG Fc antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 23.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

 

前沿进展

 
货号/价格

ILA-H5258-100ug / ¥2500

ILA-H5258-1mg (500ug X 2) / ¥16000

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