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Biotinylated Human Growth Hormone R (GHR) Protein, His Tag, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

GHR,GHBP,GH receptor

表达区间及表达系统(Source)

MABSol® Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). It is the biotinylated form of Human Growth Hormone R (GHR) Protein (GHR-H5222).

Predicted N-terminus: Ala 27

蛋白结构(Molecular Characterization)

Online(Ala 27 - Tyr 264) P10912-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 4-5 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) (Cat. No. GHR-H8222) SDS-PAGE gel

Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Biotinylated Human GHR, His Tag, ultra sensitivity (primary amine labeling) (Cat. No. GHR-H8222) with a linear range of 0.5-8 ng/mL (QC tested).

Protocol

背景(Background)

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

 

前沿进展

 
货号/价格

GHR-H8222-50ug / ¥4100

GHR-H8222-200ug / ¥12500

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Growth Hormone R靶点信息
英文全称:Growth hormone receptor
中文全称:生长激素受体
种类:Homo sapiens
上市药物数量:24详情
临床药物数量:45详情
最高研发阶段:批准上市
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