Human IL-6 R alpha, His Tag (ILR-H4223) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).
Predicted N-terminus: Leu 20
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
Immobilized ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) with a linear range of 1-16 ng/mL (QC tested).
Loaded Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) on HIS1K Biosensor, can bind ActiveMax® Human IL-6, Tag Free(Cat. No. IL6-H4218) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Tanemura Y, et al.
Journal: Biology (Basel) 2015
Application: Pulldown assay
Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126) , is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.