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 >  Protein>IL-5 R alpha >ILA-H52H4

Human IL-5 R alpha / CD125 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL5Ra,CD125

表达区间及表达系统(Source)

Human IL-5 R alpha, His Tag (ILA-H52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555 ).

Predicted N-terminus: Asp 21

Request for sequence

蛋白结构(Molecular Characterization)

IL-5 R alpha Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.7 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-5 R alpha SDS-PAGE

Human IL-5 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

IL-5 R alpha SEC-MALS

The purity of Human IL-5 R alpha, His Tag (Cat. No. ILA-H52H4) is more than 90% and the molecular weight of this protein is around 48-63 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-BLI

IL-5 R alpha BLI

Loaded Biotinylated Human IL-5, His,Avitag (Cat. No. IL5-H82Q5) on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. ILA-H52H4) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Protocol

 

背景(Background)

Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.

 

前沿进展

 
 
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IL-5 R alpha靶点信息
英文全称:Interleukin-5 receptor subunit alpha
中文全称:白细胞介素-5受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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