Human IL-21 R, His Tag (ILR-H5226) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # AAH04348).
Predicted N-terminus: Cys 20
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.8 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IL-21 R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
Immobilized Biotinylated Human IL-21, Fc,Avitag (Cat. No. IL1-H82F7) at 2 μg/mL (100 μL/well) can bind Human IL-21 R, His Tag (Cat. No. ILR-H5226) with a linear range of 2-8 ng/mL (QC tested).
Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.