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Biotinylated Human GPA33 Protein, His Tag, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

GPA33,A33

表达区间及表达系统(Source)

MABSol® Biotinylated Human GPA33, His Tag, primary amine labeling (GP3-H8224) is expressed from human HEK293 cells. It contains AA Ile 22 - Val 235 (Accession # AAH74830). It is the biotinylated form of Human GPA33, His Tag (GP3-H5224).

Predicted N-terminus: Ile 22

蛋白结构(Molecular Characterization)

Online(Ile 22 - Val 235) AAH74830

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.4 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 1-2 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human GPA33, His Tag, primary amine labeling (Cat. No. GP3-H8224) SDS-PAGE gel

Biotinylated Human GPA33, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human GPA33, His Tag, primary amine labeling (Cat. No. GP3-H8224) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human GPA33 Antibody, Human IgG1 with a linear range of 0.3-1 ng/mL (QC tested).

Protocol

背景(Background)

Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

 

前沿进展

 
货号/价格

GP3-H8224-25ug / ¥2900

GP3-H8224-200ug / ¥12500

开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
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GPA33靶点信息
英文全称:Cell surface A33 antigen
中文全称:细胞表面A33抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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