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Human IL-2 R beta / CD122 Protein, Fc Tag (SPR verified)

分子别名(Synonym)

IL2RB,RP5-1170K4.6,CD122,P70-75

表达区间及表达系统(Source)

Human IL-2 R beta, Fc Tag (SPR verified) (ILB-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1).

Predicted N-terminus: Ala 27

蛋白结构(Molecular Characterization)

Online(Ala 27 - Asp 239) NP_000869.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 50.7 kDa. The protein migrates as 62-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) SDS-PAGE gel

Human IL-2 R beta, Fc Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-15, Tag Free (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

Protocol

Biotinylated Human  ELISA

Immobilized Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5μg/well) plate, can bind Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) with a linear range of 0.8-13 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.6 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Biotinylated Human SPR

Report

Biotinylated Human SPR

Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free (SPR verified) (Cat. No. IL5-H4117) with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) on Protein A Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) on Protein A Biosensor, can bind Human IL-15, Tag Free (SPR verified) (Cat. No. IL5-H4117) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin-2 receptor subunit beta (IL2RB) is also known as CD122, which Belongs to the type I cytokine receptor family. Type 4 subfamily. IL2RB contains 1 fibronectin type-III domain. IL2RB / CD122 is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2.

 

前沿进展

 
货号/价格

ILB-H5253-50ug / ¥3500

ILB-H5253-1mg (500ug X 2) / ¥26000

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