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Human IL-17 RA / CD217 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R

表达区间及表达系统(Source)

Human IL-17 RA, Fc Tag (MALS verified) (ILA-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).

Predicted N-terminus: Leu 33

蛋白结构(Molecular Characterization)

Online(Leu 33 - Trp 320) Q96F46-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 60.1 kDa. The protein migrates as 71-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) SDS-PAGE gel

Human IL-17 RA, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) MALS images

The purity of Human IL-17 RA, Fc Tag (Cat. No.ILA-H5257) was more than 85% and the molecular weight of this protein is around 150-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 5-156 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.5-4 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free (Cat. No. ILA-H5118) with an affinity constant of 0.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) with an affinity constant of 34.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

 

前沿进展

 
货号/价格

ILA-H5257-200ug / ¥2600

ILA-H5257-1mg (500ug X 2) / ¥9800

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