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Human IL-13 R alpha 1 Protein, His Tag (SPR verified)

分子别名(Synonym)

IL13RA1,CD213A1,IL-13Ra,NR4,RP13-128O4.2,IL13Rα1

表达区间及表达系统(Source)

Human IL-13 R alpha 1, His Tag (SPR verified) (IL1-H5224) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1).

Predicted N-terminus: Gly 22

蛋白结构(Molecular Characterization)

Online(Gly 22 - Thr 343) NP_001551.1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.7 kDa. The protein migrates as 47-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>98% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human IL-13 R alpha 1, His Tag (SPR verified) (Cat. No. IL1-H5224) SDS-PAGE gel

Human IL-13 R alpha 1, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-13 R alpha 1, His Tag (SPR verified) (Cat. No. IL1-H5224) captured on CM5 chip via anti-His antibody can bind Human IL-13 with an affinity constant of 16 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

 

背景(Background)

Interleukin 13 receptor, alpha 1 is also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

 

前沿进展

 
货号/价格

IL1-H5224-100ug / ¥3150

IL1-H5224-1mg (250ug X 4) / ¥18000

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