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 >  Protein>FcRn (FCGRT & B2M) >FCM-H8286

Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (HPLC & SPR verified)

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分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (FCM-H8286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)). It is the biotinylated form of Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (FCM-H5286).

Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

Request for sequence

蛋白结构(Molecular Characterization)

FcRn (FCGRT & B2M) Structure

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FcRn (FCGRT & B2M) SDS-PAGE

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

FcRn (FCGRT & B2M) SEC-HPLC

The purity of Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) was greater than 90% as determined by SEC-HPLC.

 

活性(Bioactivity)-SPR

FcRn (FCGRT & B2M) SPR

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) captured on Biotin CAP - Series S sensor Chip can bind Herceptin® with an affinity constant of 0.57 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

FcRn (FCGRT & B2M) BLI

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) on SA Biosensor, can bind Herceptin with an affinity constant of 0.36 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

FcRn (FCGRT & B2M) BLI

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (Cat. No. FCM-H8286) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.263 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

 

前沿进展

 
 
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FcRn (FCGRT & B2M)靶点信息
英文全称:IgG receptor FcRn large subunit p51
中文全称:IgG受体FcRn大亚基p51
种类:Homo sapiens
上市药物数量:3详情
临床药物数量:3详情
最高研发阶段:批准上市
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