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 >  Fc受体蛋白>Biotinylated Human FcRn/FCGRT & B2M, ultra sensitivity (primary amine labeling)
货号/价格

FCM-H8286-25ug / ¥2900

FCM-H8286-200ug / ¥12500

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Biotinylated Human FcRn/FCGRT & B2M, ultra sensitivity (primary amine labeling)

分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

MABSol® Biotinylated Human FcRn / FCGRT & B2M (FCM-H8286) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)). It is the biotinylated form of Human FcRn / FCGRT & B2M Heterodimer Protein (Cat # FCM-H5286).

Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

蛋白结构(Molecular Characterization)

FcRn(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_001129491.1 (FCGRT) & AAH32589 (B2M)

The subunit FCGRT carries a polyhistidine tag at the C-terminus. The subunit B2M carries a Strep II tag at the C-terminus. The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

生物素标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

生物素-蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 3-5 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human FcRn / FCGRT & B2M (Cat. No. FCM-H8286) SDS-PAGE gel

Biotinylated Human FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)

Biotinylated Human FcRn / FCGRT & B2M (Catalog # FCM-H8286) ELISA bioactivity

Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human FcRn / FCGRT & B2M (Cat. No. FCM-H8286 ) with a linear range of 0.02-0.15 μg/mL (QC tested).

点击申请Protocol

Biotinylated Human FcRn Heterodimer Protein BLI

Immobilized Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein (Cat. No. FCM-H8286) on SA Biosensor, can bind Herceptin with an affinity constant of 0.49 μM as determined in BLI assay (Fortebio Octet 96) ( Routinely tested).

点击申请Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

参考文献(References)

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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