Human IFN-gamma R1, Fc Tag (IF1-H5254) is expressed from human 293 cells (HEK293). It contains AA Glu 18 - Gly 245 (Accession # AAH05333).
Predicted N-terminus: Glu 18
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.4 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IFN-gamma R1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. IF1-H5254) with a linear range of 1-10 ng/mL (QC tested).
Interferon gamma receptor 1 (IFNGR1) is also known as CD antigen CD119, which belongs to the type II cytokine receptor family. IFNGR1 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. IFNGR1 / CD119 is receptor for interferon gamma. Two receptors bind one interferon gamma (IFNG) dimer.