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Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein (SPR verified)

分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Biotinylated Human FcRn Heterodimer Protein (SPR verified) (FCM-H82W4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).

Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

蛋白结构(Molecular Characterization)

Online(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) P55899-1 (FCGRT) & P61769-1 (B2M)

Biotinylated Human FcRn Heterodimer Protein (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human FcRn Heterodimer Protein (SPR verified) (Cat. No. FCM-H82W4) SDS-PAGE gel

Biotinylated Human FcRn Heterodimer Protein (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Yervoy Ipilimumab at 10 μg/mL (100 μL/well) can bind Biotinylated Human FcRn Heterodimer Protein (SPR verified) (Cat. No. FCM-H82W4) with a linear range of 20-625 ng/mL (QC tested).

点击申请Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human FcRn Heterodimer Protein (SPR verified) (Cat. No. FCM-H82W4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).

点击申请Protocol

 

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

 

专利使用说明(Limited Use License)

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

 

前沿进展

货号/价格

FCM-H82W4-25ug / ¥3500

FCM-H82W4-200ug / ¥13800

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