膜杰作
Star Staining
分子别名（Synonym）
GLRX,GRX,TTase-1,GLRX1,GRX1
表达区间及表达系统（Source）
Human Glutaredoxin 1, His Tag (GLX-H5149) is expressed from E. coli cells. It contains AA Met 1 - Gln 106 (Accession # NP_001112362).
Predicted N-terminus: Met 1
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 12.7 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with 1 mM DTT. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Glutaredoxin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
文献引用（Citations）
Authors: Barinova KV, et al.
Journal: Biochim Biophys Acta 2017
Application:
背景（Background）
Glutaredoxin-1 (GRX1) is also known as Thioltransferase-1 (TTase-1), GLRX, GRX, GLRX1, which belongs to the glutaredoxin family. GRX1 / GLRX contains one glutaredoxin domain, which exists in either a reduced or an oxidized form. GRX1 / GLRX has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase and functions as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.
