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Human FGF R1 / CD331 Protein, His Tag

分子别名(Synonym)

FGFR1,BFGFR,CD331,CEK,FGFBR,FLG,HBGFR,N-SAM,FLT2,H2,KAL2,FLJ14326

表达区间及表达系统(Source)

Human FGF R1, His Tag (FG1-H5223) is expressed from human 293 cells (HEK293). It contains AA Arg 22 - Ile 376 (Accession # P11362-7).

Predicted N-terminus: Arg 22

蛋白结构(Molecular Characterization)

Online(Arg 22 - Ile 376) P11362-7

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 41.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human FGF R1, His Tag (Cat. No. FG1-H5223) SDS-PAGE gel

Human FGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with a linear range of 0.156-1.25 μg/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human FGF R1, His Tag (Cat. No. FG1-H5223) on HIS1K Biosensor, can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 0.16 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Fibroblast growth factor receptor 1 (FGFR1) is also known as basic fibroblast growth factor receptor 1(BFGFR1), FMS-like tyrosine kinase, CD331, and is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. This protein is one of several fibroblast growth factor receptors, which are related proteins that are involved in important processes such as cell division, regulation of cell growth and maturation, formation of blood vessels, wound healing, and embryonic development.The FGFR1 protein spans the cell membrane, so that one end of the protein remains inside the cell and the other end projects from the outer surface of the cell. This positioning allows the FGFR1 protein to interact with specific growth factors outside the cell and to receive signals that help the cell respond to its environment. When growth factors attach to the FGFR1 protein, the receptor triggers a cascade of chemical reactions inside the cell that instruct the cell to undergo certain changes, such as maturing to take on specialized functions. The FGFR1 protein is thought to play an important role in the development of the nervous system. This protein may also help regulate the growth of long bones, such as the large bones in the arms and legs.

 

前沿进展

 
货号/价格

FG1-H5223-200ug / ¥3500

FG1-H5223-1mg / ¥11000

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