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 >  Fc受体蛋白>Human FcRn/FCGRT & B2M Heterodimer Protein
货号/价格

FCM-H5286-50ug / ¥3500

FCM-H5286-1mg (500ug x 2) / ¥24000

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Human FcRn/FCGRT & B2M Heterodimer Protein

分子别名(Synonym)

FcRn,FCGRT & B2M

表达区间及表达系统(Source)

Human FcRn / FCGRT & B2M heterodimer protein, Ala 24 - Ser 297 (FCGRT, NP_001129491.1) and & Ile 21 - Met 119 (B2M, AAH32589), was produced in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

Request for sequence

蛋白结构(Molecular Characterization)

FcRn(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) NP_001129491.1 (FCGRT) & AAH32589 (B2M)

Human FcRn / FCGRT & B2M heterodimer protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa FCGRT and 13.1 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The predicted N-terminus is Ala 24(FCGRT) & Ile 21(B2M). The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human FcRn / FCGRT & B2M (Cat. No. FCM-H5286) SDS-PAGE gel

Human FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)

Human FcRn / FCGRT & B2M (Cat. No. FCM-H5286) ELISA bioactivity

Immobilized Anti-CTLA4 MAb, Human IgG1 at 10 μg/mL (100 μL/well) can bind Human FcRn, His Tag, Strep Tag (Cat. No. FCM-H5286) with a linear range of 0.156-1.25 μg/mL (QC tested).

点击申请Protocol

Human FcRn Heterodimer Protein SPR

Immobilized Herceptin on CM5 Chip, can bind Human FcRn / FCGRT & B2M Heterodimer Protein(Cat. No. FCM-H5286) with an affinity constant of 0.92 μM as determined in SPR assay (Biacore 8K) ( Routinely tested).

点击申请Protocol

Human FcRn Heterodimer Protein SPR

Immobilized Human FcRn / FCGRT & B2M Heterodimer Protein(Cat. No. FCM-H5286) on CM5 Chip, can bind Herceptin with an affinity constant of 0.427 μM as determined in SPR assay (Biacore 8K) ( Routinely tested).

点击申请Protocol

Human FcRn Heterodimer Protein BLI

Immobilized Herceptin on SA Biosensor via Biotinylated Her2 Protein (Cat. No. HE2-H822R), can bind Human FcRn / FCGRT & B2M Heterodimer Protein (Cat. No. FCM-H5286) with an affinity constant of 1.32 μM as determined in BLI assay (Fortebio Octet 96) ( Routinely tested).

点击申请Protocol

Human FcRn Heterodimer Protein BLI

 Immobilized Human FcRn / FCGRT & B2M Heterodimer Protein (Cat. No. FCM-H5286) on His2 Biosensor, can bind Herceptin with an affinity constant of 0.44 μM as determined in BLI assay (Fortebio Octet 96) ( Routinely tested).

点击申请Protocol

 

文献引用(Citations)

背景(Background)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

参考文献(References)

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