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Human Fas / TNFRSF6 / CD95 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

FAS,ALPS1A,APO1,APT1,CD95,FAS1,FASTM,TNFRSF6,FasR

表达区间及表达系统(Source)

Human Fas Protein, Fc Tag (MALS verified) (FAS-H5252) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Asn 173 (Accession # AAH12479.1).

Predicted N-terminus: Gln 26

蛋白结构(Molecular Characterization)

Online(Gln 26 - Asn 173) AAH12479.1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 42.8 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Fas Protein, Fc Tag (MALS verified) (Cat. No. FAS-H5252) SDS-PAGE gel

Human Fas Protein, Fc Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human Fas Protein, Fc Tag (MALS verified) (Cat. No. FAS-H5252) MALS images

The purity of Human Fas Protein, Fc Tag (MALS verified) (Cat. No.FAS-H5252) was more than 90% and the molecular weight of this protein is around 93-110 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Fas Ligand, His Tag (Cat. No. FAL-H5241) at 2 μg/mL (100 μL/well)can bind Human Fas, Fc Tag (Cat. No. FAS-H5252) with a linear range of 4-31 ng/mL (QC tested).

Protocol

背景(Background)

The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.

 

前沿进展

 
货号/价格

FAS-H5252-100ug / ¥2600

FAS-H5252-1mg / ¥14000

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Fas靶点信息
英文全称:Apoptosis-mediating surface antigen FAS
中文全称:Fas抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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