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 >  Protein>Fas >FAS-H5229

Human Fas / TNFRSF6 / CD95 Protein, His Tag (MALS verified)

分子别名(Synonym)

FAS,ALPS1A,APO1,APT1,CD95,FAS1,FASTM,TNFRSF6,FasR

表达区间及表达系统(Source)

Human Fas, His Tag (FAS-H5229) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Asn 173 (Accession # P25445-1).

Predicted N-terminus: Gln 26

Request for sequence

蛋白结构(Molecular Characterization)

Fas Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Fas SDS-PAGE

Human Fas, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Fas SEC-MALS

The purity of Human Fas, His Tag (Cat. No. FAS-H5229) is more than 85% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

Report

 

背景(Background)

The Fas is also known as FAS receptor (FasR), apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6). is a death receptor on the surface of cells that leads to programmed cell death (apoptosis). It is one of two apoptosis pathways, the other being the mitochondrial pathway. FasR is located on chromosome 10 in humans and 19 in mice. Similar sequences related by evolution (orthologs) are found in most mammals. Fas forms the death-inducing signaling complex (DISC) upon ligand binding. Membrane-anchored Fas ligand trimer on the surface of an adjacent cell causes trimerization of Fas receptor. This event is also mimicked by binding of an agonistic Fas antibody, though some evidence suggests that the apoptotic signal induced by the antibody is unreliable in the study of Fas signaling. To this end, several clever ways of trimerizing the antibody for in vitro research have been employed.Upon ensuing death domain (DD) aggregation, the receptor complex is internalized via the cellular endosomal machinery. This allows the adaptor molecule FADD to bind the death domain of Fas through its own death domain. Recently, Fas has also been shown to promote tumor growth, since during tumor progression, it is frequently downregulated or cells are rendered apoptosis resistant. Cancer cells in general, regardless of their Fas apoptosis sensitivity, depend on constitutive activity of Fas. This is stimulated by cancer-produced Fas ligand for optimal growth.

 

前沿进展

 
 
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Fas靶点信息
英文全称:Apoptosis-mediating surface antigen FAS
中文全称:Fas抗原
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
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