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Human E-Selectin / CD62E Protein, His Tag

分子别名(Synonym)

SELE,RP1-117P20.2,CD62E,ELAM1,ESEL,LECAM2,E-Selectin

表达区间及表达系统(Source)

Human E-Selectin, His Tag (CDE-H5222) is expressed from human 293 cells (HEK293). It contains AA Trp 22 - Pro 556 (Accession # NP_000441.2).

Predicted N-terminus: Trp 22

蛋白结构(Molecular Characterization)

Online(Trp 22 - Pro 556) NP_000441.2

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.5 kDa. The protein migrates as 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human E-Selectin, His Tag (Cat. No. CDE-H5222) SDS-PAGE gel

Human E-Selectin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

背景(Background)

E-Selectin is also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1β and TNFα. E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto). During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The local release of cytokines IL-1 and TNF-α by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels. E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

 

前沿进展

 
货号/价格

CDE-H5222-200ug / ¥3000

CDE-H5222-1mg (250ug X 4) / ¥12000

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E-Selectin靶点信息
英文全称:Selectin E
中文全称:选择素-E
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床三期
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