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Human ErbB3 / Her3 Protein, Fc Tag

分子别名(Synonym)

ERBB3,HER3,LCCS2,MDA-BF-1,MGC88033,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3

表达区间及表达系统(Source)

Human ErbB3, Fc Tag (ER3-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # NP_001973.2).

Predicted N-terminus: Ser 20

蛋白结构(Molecular Characterization)

Online(Ser 20 - Thr 643) NP_001973.2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 94.8 kDa. The protein migrates as 116-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human ErbB3, Fc Tag (Cat. No. ER3-H5259) SDS-PAGE gel

Human ErbB3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human ErbB3, Fc Tag (Cat. No. ER3-H5259) at 2 μg/mL (100 μL/well) can bind Human NRG1-beta 1 Protein with a linear range of 1-20 ng/mL (Routinely tested).

Protocol

背景(Background)

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

 

前沿进展

 
货号/价格

ER3-H5259-200ug / ¥3400

ER3-H5259-1mg (500ug X 2) / ¥14400

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ErbB3靶点信息
英文全称:Receptor tyrosine-protein kinase erbB-3
中文全称:受体酪氨酸蛋白激酶erbB-3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:29详情
最高研发阶段:临床三期
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