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Human DLL4 Protein, His Tag

分子别名(Synonym)

DLL4,Delta4

表达区间及表达系统(Source)

Human DLL4, His Tag (DL4-H5227) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # Q9NR61-1).

Predicted N-terminus: Ser 27

蛋白结构(Molecular Characterization)

Online(Ser 27 - Pro 524) Q9NR61-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 55.5 kDa. The protein migrates as 58-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human DLL4, His Tag (Cat. No. DL4-H5227) SDS-PAGE gel

Human DLL4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human DLL4 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Mouse Notch-1 Protein ECD, Fc Tag at 2 μg/mL (100 μL/well) can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with a linear range of 0.039-0.313 μg/mL (Routinely tested).

Protocol

背景(Background)

Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.

 

前沿进展

 
货号/价格

DL4-H5227-100ug / ¥2500

DL4-H5227-1mg (250ug X 4) / ¥14000

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DLL4靶点信息
英文全称:Delta-like protein 4
中文全称:δ样蛋白-4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:7详情
最高研发阶段:临床二期
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