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Human DcR3 / TNFRSF6B Protein, Fc Tag

分子别名(Synonym)

TNFRSF6B,DCR3,TR6,M68

表达区间及表达系统(Source)

Human DcR3, Fc Tag (TNB-H5255) is expressed from human 293 cells (HEK293). It contains AA Val 30 - His 300 (Accession # O95407-1).

Predicted N-terminus: Val 30

蛋白结构(Molecular Characterization)

Online(Val 30 - His 300) O95407-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 56.2 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human DcR3, Fc Tag (Cat. No. TNB-H5255) SDS-PAGE gel

Human DcR3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 5 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 5-20 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human DcR3, Fc Tag(Cat. No. TNB-H5255)on AHC Biosensor, can bind Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin(Cat. No. LIT-H5256)with an affinity constant of 1.64 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human DcR3, Fc Tag (Cat. No. TNB-H5255) on AHC Biosensor, can bind Human LIGHT, His Tag (Cat. No. LIT-H5247) with an affinity constant of 4.94 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) is also known as Decoy Receptor 3 (DcR3), Decoy receptor for Fas ligand and M68, which belongs to the tumor necrosis factor receptor superfamily. TNFRSF6 is a soluble protein which contains four TNFR-Cys repeats. Unlike most of the other members of TNFR superfamily, TNFRSF6 is a soluble protein which contains no transmembrane domain. TNFRSF6B acts as a decoy receptor that competes with death receptors for ligand binding. The protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A.

 

前沿进展

货号/价格 货号/价格

TNB-H5255-50ug / ¥3150

TNB-H5255-1mg (500ug X 2) / ¥25000

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