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Human CD58 / LFA-3 Protein, His Tag

分子别名(Synonym)

CD58,LFA3,Ag3

表达区间及表达系统(Source)

Human CD58, His Tag (LF3-H5225) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Arg 215 (Accession # AAH05930).

Predicted N-terminus: Phe 29

蛋白结构(Molecular Characterization)

Online(Phe 29  -  Arg 215) AAH05930

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD58, His Tag (Cat. No. LF3-H5225) SDS-PAGE gel

Human CD58, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD2, Fc Tag (Cat. No. CD2-H5258) at 10 μg/mL (100 μL/well) can bind Human CD58, His Tag (Cat. No. LF3-H5225) with a linear range of 0.078-2.5 μg/mL (QC tested).

Protocol

背景(Background)

CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.

 

前沿进展

 
货号/价格

LF3-H5225-100ug / ¥2800

LF3-H5225-1mg / ¥16000

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