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Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

分子别名(Synonym)

CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon

表达区间及表达系统(Source)

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-H52W0) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

蛋白结构(Molecular Characterization)

Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) heterodimer protein migrates as 40-55 kDa due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) SDS-PAGE gel

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) MALS images

The purity of Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) was more than 90% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) at 2 μg/mL (100 μL/well) can bind OKT3 (mouse IgG2a) (Cat. No. CDE-M120a) with a linear range of 10-78 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W0) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind OKT3 (mouse IgG2a) with an affinity constant of 31.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

前沿进展

 
货号/价格

CDD-H52W0-100ug / ¥2900

CDD-H52W0-1mg / ¥17600

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