Human CD160, His Tag (BY5-H5229) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Ser 159 (Accession # O95971-1).
Predicted N-terminus: Ile 27
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.7 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD160, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human HVEM, Fc Tag (Cat. No. HV4-H82F1) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5μg/well) plate, can bind Human CD160, His Tag (Cat. No. BY5-H5229) with a linear range of 8-63 ng/mL (QC tested).
CD160 antigen is also known as natural killer cell receptor BY55, is a 27 kDa glycoprotein, contains 1 Ig-like V-type (immunoglobulin-like) domain. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.