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 >  Protein>CD3E & CD3G >CDG-C8219

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

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分子别名(Synonym)

CD3 epsilon & CD3 gamma,CD3E & CD3G

表达区间及表达系统(Source)

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-C8219) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)).

Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)

Request for sequence

蛋白结构(Molecular Characterization)

CD3E & CD3G Structure

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 17.7 kDa (CD3E) and 15.4 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-20 kDa and 22-24 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD3E & CD3G SDS-PAGE

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

CD3E & CD3G SEC-MALS

The purity of Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) is more than 90% and the molecular weight of this protein is around 33-48kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD3E & CD3G ELISA

Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No.CDG-C8219) with a linear range of 0.98-15.6 ng/mL (QC tested).

Protocol

 

背景(Background)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

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